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ÕªÒª£º±¾ÎÄÆÀÊöÁ˸ßЧҺÏàÉ«Æ×£¨HPLC£©¶ÔʳƷÖÐËÕµ¤ºì¼ì²âµÄÑо¿½øÕ¹£¬ÄÚÈÝ×ÅÖØ°üÀ¨ÑùÆ·µÄÖƱ¸¡¢ÑùÆ·Ô¤´¦Àí¡¢¾»»¯¡¢ÝÍÈ¡¼ÁµÄÑ¡Ôñ¡¢¼ì²â²¨³¤µÄÑ¡Ôñ¡¢É«Æ×¼ì²âÆ÷µÄÑ¡ÔñµÈ¡£ÊµÑ鲿·Ö£¬²ÉÓøßЧҺÏàÉ«Æ×·¨²â¶¨¼¦µ°»ÆÖеÄËÕµ¤ºì¢ñ¡¢ËÕµ¤ºì¢ò¡¢ËÕµ¤ºì¢ó¡¢ËÕµ¤ºì¢ô¡£ÒÔÒÒëæÌáÈ¡¼¦µ°»ÆÖеÄËÕµ¤ºì£¬ÒÔ¼×Ëá¡¢ÒÒëæ¡¢±ûͪºÍË®°´Ò»¶¨±ä»¯µÄ±ÈÂÊ»ìºÏ×÷ΪÁ÷¶¯Ï࣬¾Zorbox-C18É«Æ×Öù£¨4.6mm¡Á150mm£©·ÖÀ룬²ÉÓÃ478nm£¬520nm¶þ¶Î±ä²¨³¤¼ì²â£¬»ñµÃÁ˽ϺõķÖÀëЧ¹ûºÍ½ÏµÍµÄ¼ì²âÏÞ¡£¸Ã·¨ÓÃÓÚ¼¦µ°ÖÐËÕµ¤ºìµÄ²â¶¨£¬ËÕµ¤ºì¢ñ¡¢ËÕµ¤ºì¢ò¡¢ËÕµ¤ºì¢ó¡¢ËÕµ¤ºì¢ôµÄ»ØÊÕÂÊ·Ö±ðΪ85%¡¢90%¡¢92%¡¢95%£¬Ïà¶Ô±ê׼ƫ²î·Ö±ðΪ2.7%¡¢4.3%¡¢5.3%¡¢3.5%¡£¸Ã·¨¼ò±ã¡¢¿ìËÙ¡¢×¼È·£¬Äܹ»Âú×ãʵ¼ÊµÄ¼ì²âÐèÒª¡£
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Abstract: In this paper£¬the progress on determination of Sudan dyes by HPLC were reviewed. The contents include the pretreatment and purification of sample£¬the selection of extraction agent of Sudan dyes£¬wavelength£¬mobile phase£¬chromatographic detector£¬and so on. In the part of experiment£¬ the content of Sudan¢ñ¡¢Sudan¢ò¡¢Sudan¢ó¡¢Sudan¢ô in chick egg yolk has been determined with a high performance liquid chromatographic method. These Sudan dyes were extracted with acetonitrile. The Sudan dyes were separated on a ZorboxSB-C18 column(4.6mm¡Á150mm) with a mixture of acetonitrile¡¢acetone¡¢formic acid and water in certain varied ratio as mobile phase. They were then detected at two shifted wavelength 478nm-520nm£¬with it the experiment gains good effect for separation and low detecting limit. The recoveries of Sudan¢ñ¡¢Sudan¢ò¡¢Sudan¢ó¡¢Sudan¢ô were 85%¡¢90%¡¢92%¡¢95% respectively. The relative standard deviations were 2.7%¡¢4.3%¡¢5.3%¡¢3.5% respectively. The method is simple, rapid and accurate£¬and can meet the needs of practical determination entirely.
Key words: Foodstuff; Determination; Sudan Dyes; Extraction; High Performance Liquid
Chromatographic