发布时间：2015-04-01 来源：人大经济论坛

制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 概述1 1.2 茶皂素的理化特性1 1.3 茶皂素的实际用途1 1.3.1 茶皂素的溶血作用及鱼毒作用1 1.3.2 茶皂素在医药上的应用2 1.3.3 工业上的应用2-3 1.3.4 农业上的应用3 2. 实验部分4 2.1 试剂与仪器4 2.1.1 实验材料4 2.1.2 主要试剂4 2.1.3 主要仪器设备4 2.2 实验步骤5 2.2.1实验中溶液的准备5 2.2.2含量80%粗提取物的精制5 2.2.3供试品溶液的配制5-6 2.2.4操作条件6-7 2.2.5含量的测定7-10 3. 结果与讨论10 3.1 精制过程中PH和温度的控制 10 3.2 讨论10-12 4．总结与展望12 4.1 实验总结12 4.2 展望12-13 致谢14 参考文献15 摘要：本文综述了茶皂素的性质、提取精制及油茶皂素定量方法的研究，并总结了当前茶皂素研究中最值得进一步研究的问题。对粗茶皂素的提取纯化工艺进行了研究。以质量分数为70％的粗茶皂素为原料，经2％ NaOH溶解、盐酸酸析、95％ 乙醇溶解、丙酮沉析工艺得到精制茶皂素。研究了用反相高效液相色谱法(HPLC)测定茶皂素含量的方法，以Hewlett Packard ODS Hypersil 5 微米，125×4毫米不锈钢柱作分析柱，以甲醇作流动相，流速控制在0.5mL／min，检测波长276nm。通过反相高效液相色谱法测定经过精制后的茶皂素的含量为97.6709％。 关键词：反向高效液相色谱；茶皂素；提取纯化 Abstract：This paper summarizes the development of the property, extraction and purification, and quantitative determination of tea saponin. Besides, issues needing further study were indicated .A simple purification method for crude tea saponin was described. The 70%of crude tea saponin was dissolved in 2% sodium hydroxide aqueous solution and precipitated by adding 20% hydrochloric acid. The deposit was dissolved with 95%ethanol, and the acetone was added to the ethanol solution to make the tea saponin to be precipitated. A reverse phase high performance liquid chromatography (HPLC) method for determination of tea saponin extracted from tea-seed pancake with Soxhlet was studied. With the column Hewlett Packard ODS Hypersil (5μm 125mm*4mm)and mobile phase methanol at flow-rate of 0.5 ml/min. The recovery rate of tea saponin was 97.6709% Key words：reverse-phase high perform ance liquid chromat0graphy;tea saponin; purification