液体全麻降低血浆临界温度 膜囊泡

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摘要翻译：
大量不同的小疏水分子引起全身麻醉。它们作为麻醉药的功效已被证明与它们对疏水环境的亲和力和它们抑制某些配体门控离子通道的效力有关。本文探讨了N-醇和其他液体麻醉药对细胞源性巨质膜囊泡（GPMVs）二维相容性临界点的影响。我们发现，麻醉药降低了这些GPMV的临界温度(Tc)，但没有强烈改变低于Tc的两个液相的比例。当N-醇的浓度用蝌蚪麻醉的中值麻醉剂浓度(AC50)重新标定时，这种影响的大小是一致的，但当与溶液中的总浓度作图时，这种影响的大小不一致。在AC50时，我们看到Tc有4{\\deg}C的下移，比这些麻醉剂浓度下主链转变的典型值大得多。异丙酚、苯乙醇和异丙醇以麻醉剂的浓度加入时，也能降低GPMV的相容性临界温度，但十四醇或2,6二丁基苯酚不能降低GPMV的相容性临界温度，十四醇或2,6二丁基苯酚是两种疏水但没有麻醉效力的全身麻醉药的结构类似物。我们认为液体全身麻醉药为降低完整细胞质膜的临界温度提供了一个实验工具，我们预测这将以增加或减少胆固醇的方式减少脂质介导的异质性。此外，我们的结果的几个可能的含义被讨论在当前的麻醉作用于配体门控离子通道的模型的背景下。
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英文标题：
《Liquid general anesthetics lower critical temperatures in plasma
  membrane vesicles》
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作者：
Ellyn Gray, Joshua Karslake, Benjamin B. Machta, Sarah L. Veatch
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最新提交年份：
2013
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分类信息：

一级分类：Physics        物理学
二级分类：Biological Physics        生物物理学
分类描述：Molecular biophysics, cellular biophysics, neurological biophysics, membrane biophysics, single-molecule biophysics, ecological biophysics, quantum phenomena in biological systems (quantum biophysics), theoretical biophysics, molecular dynamics/modeling and simulation, game theory, biomechanics, bioinformatics, microorganisms, virology, evolution, biophysical methods.
分子生物物理、细胞生物物理、神经生物物理、膜生物物理、单分子生物物理、生态生物物理、生物系统中的量子现象（量子生物物理）、理论生物物理、分子动力学/建模与模拟、博弈论、生物力学、生物信息学、微生物、病毒学、进化论、生物物理方法。
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一级分类：Physics        物理学
二级分类：Soft Condensed Matter        软凝聚态物质
分类描述：Membranes, polymers, liquid crystals, glasses, colloids, granular matter
膜，聚合物，液晶，玻璃，胶体，颗粒物质
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一级分类：Quantitative Biology        数量生物学
二级分类：Biomolecules        生物分子
分类描述：DNA, RNA, proteins, lipids, etc.; molecular structures and folding kinetics; molecular interactions; single-molecule manipulation.
DNA、RNA、蛋白质、脂类等；分子结构与折叠动力学；分子相互作用；单分子操作。
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一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
--

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英文摘要：
  A large and diverse array of small hydrophobic molecules induce general anesthesia. Their efficacy as anesthetics has been shown to correlate both with their affinity for a hydrophobic environment and with their potency in inhibiting certain ligand gated ion channels. Here we explore the effects that n-alcohols and other liquid anesthetics have on the two-dimensional miscibility critical point observed in cell derived giant plasma membrane vesicles (GPMVs). We show that anesthetics depress the critical temperature (Tc) of these GPMVs without strongly altering the ratio of the two liquid phases found below Tc. The magnitude of this affect is consistent across n-alcohols when their concentration is rescaled by the median anesthetic concentration (AC50) for tadpole anesthesia, but not when plotted against the overall concentration in solution. At AC50 we see a 4{\deg}C downward shift in Tc, much larger than is typically seen in the main chain transition at these anesthetic concentrations. GPMV miscibility critical temperatures are also lowered to a similar extent by propofol, phenylethanol, and isopropanol when added at anesthetic concentrations, but not by tetradecanol or 2,6 diterbutylphenol, two structural analogs of general anesthetics that are hydrophobic but have no anesthetic potency. We propose that liquid general anesthetics provide an experimental tool for lowering critical temperatures in plasma membranes of intact cells, which we predict will reduce lipid-mediated heterogeneity in a way that is complimentary to increasing or decreasing cholesterol. Also, several possible implications of our results are discussed in the context of current models of anesthetic action on ligand gated ion channels.
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PDF链接：
https://arxiv.org/pdf/1309.2684

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关键词：Quantitative interactions manipulation Implications Experimental liquid critical AC50 典型值 临界温度