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摘要翻译：
AMP激活的蛋白激酶(AMPK)是一种重要的能量传感器，调节着包括代谢性疾病和癌症在内的病理过程中的信号网络。AMPK的作用日益被人们所认识，这促使大量的研究努力开发新的药理学AMPK激活剂。为了准确地研究AMPK的作用，以及AMPK激活剂在细胞模型中的特异性和活性，需要遗传性AMPK灭活工具。本文报道了利用CRISPR/Cas9技术对人类细胞系中AMPK$\alpha1/\alpha2$催化亚基进行基因失活的方法。
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英文标题：
《Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system》
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作者：
Adrien Grenier, Pierre Sujobert (CRCL), S\'everine Olivier (IC UM3
(UMR 8104 / U1016)), H\'el\`ene Guermouche, Johanna Mondesir, Olivier
Kosmider (IC UM3 (UMR 8104 / U1016)), Benoit Viollet (EMD), J\'er\^ome
Tamburini (IC UM3 (UMR 8104 / U1016))
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最新提交年份：
2019
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分类信息：
一级分类：Quantitative Biology 数量生物学
二级分类：Other Quantitative Biology 其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
AMP activated protein kinase (AMPK) is a critical energy sensor, regulating signaling networks involved in pathology including metabolic diseases and cancer. This increasingly recognized role of AMPK has prompted tremendous research efforts to develop new pharmacological AMPK activators. To precisely study the role of AMPK, and the specificity and activity of AMPK activators in cellular models, genetic AMPK inactivating tools are required. We report here methods for genetic inactivation of AMPK $\alpha1/ \alpha2$ catalytic subunits in human cell lines by the CRISPR/Cas9 technology, a recent breakthrough technique for genome editing.
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PDF链接：
https://arxiv.org/pdf/1911.02346
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