In this paper, the conception of effective coopetition mechanism in high quality pork supply chain has been analyzed. The evolutional processes of co-opetition mechanism and related influencing factors have been studied by using the dual population evolutionary game theory. The result showed that evolution of co-opetition is influenced by 8 factors, which are cooperation cost, cooperation income, coefficient of income distribution, decrease of cooperative risk, coefficient of risk compensation, probability of risk, management scale, and coefficients of either encouragement or punishment. It also suggested that the achievement of effective competition- cooperation of high quality pork supply chain would be improved by decreasing cooperation costs, increasing cooperation incomes, and building the equitable and effective systems of income distribution, risk compensation, as well as encouragement and punishment.