发布时间：2015-01-06 来源：人大经济论坛

大鼠弥漫性脑损伤后Homer蛋白的表达及其意义_药学专业毕业论文范文 作者：史铁钧，费舟，章翔，黄卫东，梁景文，宋蕾 【关键词】 弥漫性脑损伤 Expression of Homer after diffuse brain injury in rats and its significance 【Abstract】 AIM: To study the expression and significance of Homer in diffuse brain injury（DBI）of rat in vitro. METHODS: One hundred and five SD rats, weighing（300±25）g, were randomized to normal, shamoperated and DBI groups. The Marmarou rats were applied to DBI group and the shamoperated group was treated as DBI group except for “free fall” attack. The rats main brain areas and nuclei were taken to detect for Homer by immunoblotting at 0.5, 1, 6, 12, 24, 72 and 168 h after injury respectively. RESULTS: Homer1a was positive in DBI group from 0.5 h to 168 h after injury but no Homer1a was observed in normal group and shamoperated group. Homer1b/c, Homer2a/b and Homer3 were present in the normal, shamoperated and DBI groups. CONCLUSION: There is a significant change in Homer1a expression before and after injury, but there is no significant change in Homer1b/c, Homer2a/b and Homer3 levels, which indicates that Homer1a is involved in the neuronal injury and may protect neurons from insults. 【Keywords】 Homer; immunoblotting; diffuse brain injury; rats 【摘要】 目的： 研究大鼠弥漫性脑损伤后不同亚型Homer蛋白表达规律及其意义. 方法： SD大鼠105只，体质量（300±25） g，分为正常组、假手术组和弥漫性脑损伤(DBI)组， DBI组应用Marmarou大鼠致伤，假手术组不予以自由落体打击，其余处理同DBI组，分别在损伤后0.5, 1, 6, 12, 24, 72, 168 h取大鼠主要脑区及核团裂解匀浆，进行各Homer蛋白的免疫印迹分析. 结果： DBI组神经组织中Homer1a蛋白的表达自伤后0.5 h起持续至伤后168 h，而正常及假手术组未见该蛋白阳性表达；Homer1b/c, Homer2a/b和Homer3在正常组、假手术组和DBI组均可见明确的阳性表达. 结论： 大鼠弥漫性脑损伤后Homer1a蛋白的表达于损伤前后有显著变化，而Homer1b/c, Homer2a/b及Homer3于损伤前后无明显变化，提示Homer1a蛋白参与了神经元损伤过程,且可能对神经元损伤起到一定的保护作用. 【关键词】 Homer；免疫印迹法；弥漫性脑损伤；大鼠 0引言 1997年Brakeman等首次发现Homer，目前Homer家族已分为3型(Homer1,Homer2和Homer3)，其中Homer1是唯一在外来刺激后高表达的家族蛋白，每型还有多种剪切变异，如Homer1a, Homer1b和Homer1c等. Homer1a是一种即早基因（immediately early gene, IEG）产物，它在细胞中呈动态表达,而随后又发现Homer1b, Homer1c,Homer2a, Homer2b及Homer3等，通常在多种神经元的突触后膜上表达，除含有与Homer1a相同的EVH1（Ena/VASP homology 1）结构域外，还在C端包含有一段卷曲螺旋结构域. 我们研究在大鼠弥漫性脑损伤(DBI)模型基础上Homer蛋白分子的表达及其意义. 1材料和方法 1.1材料 健康成年雄性SD大鼠105只，体质量为（300±25）g，第四军医大学动物实验中心提供. 各Homer蛋白亚型的多克隆抗体均购自SantaCruz公司，不连续聚丙烯酰胺凝胶电泳（SDSPAGE）低分子量标准蛋白及BCIP/NBT显色盒购自武汉博士德公司. 1.2方法 1.2.1动物分组及模型制作SD大鼠随机分为正常组、假手术组及损伤组，其中损伤组据标本采集时间又划分为0.5, 1, 6, 12, 24, 72, 168 h等7个亚组，每组5只，即在7个时间点划入实验范围的大鼠均为15只，损伤组采用Marmarou大鼠DBI模型［1］，假手术组仅不予以自由落体打击，其余处理同损伤组.