发布时间：2015-01-24 来源：人大经济论坛

生物工程专业论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 蟑螂特征......................................................1 1.2 蟑螂真相 .....................................................1 1.3 药理作用 .....................................................2 1.4 分离提取......................................................3 1.5 化学成分......................................................3 1.6 实验目的......................................................4 2. 实验部分5 2.1 实验材料......................................................5 2.1.1 实验样品................................................5 2.2 实验准备工作..................................................5 2.3 硅胶柱层析....................................................6 2.4 硅胶薄板层析..................................................7 2.5 大孔树脂吸附..................................................7 2.6 高效液相色谱分析..............................................8 2.7 LH-20 反相层析................................................8 2.8 重复实验......................................................9 2.9 显色反应......................................................9 2.10 液质联用分析.................................................9 2.11 合并冻干.....................................................10 3. 结果与讨论.........................................................10 3.1 萃取液的液相图................................................10 3.2 KFX-1, KFX-2, K FX-3，KFX-4的分离.............................11 3.3 KFX-5的分离...................................................15 3.4 检测..........................................................18 4．总结与展望.........................................................20 致谢..................................................................22 参考文献..............................................................23 附录1(质谱图) ........................................................25 附录2(保密协议) ......................................................26 摘要：科学研究者对大蠊进行研究后发现，大蠊中含有的一些化学成分可能有一定得药物活性。本实验就是想先用乙酸乙酯对大蠊浸膏进行萃取，除去非极性部分；得到的乙酸乙酯相，用硅胶柱吸附层析，再用高效液相色谱进行分析，然后经LH-20反相层析得到4种目标物质；萃取得到的水相用大孔树脂吸附，先用20％酒精溶液洗脱除去极性部分，再用40％酒精溶液洗脱收集，然后用高效液相色谱进行分析，再用LH-20反相层析得到1种纯的目标物质；最后对分离纯化得到的5种目标物质进行显色反应、紫外光谱及液质等检测。 关键字：色谱；化学成分；大蠊；显色反应 Studies on the isolation and purification of chemical constituents from Periplaneta Abstract: Scientific researchers studied that Periplaneta contains some of the chemical constituents which may have some a drug ctivity. This experiment firstly want to use ethyl acetate to extract Periplaneta extraction, remove non-polar part. the phase of ethyl acetate, use silica gel column to carry out adsorption chromatography, and then use high-performance liquid chromatography to analyse,and then by the LH-20 reversed-phase chromatography to get four kinds of target substances; Aqueous extract obtained by macroporous resin adsorption, first with 20% alcohol solution to remove polar elution ,then with 40% alcohol solution elute and collect, and then use high-performance liquid chromatography to analyse, then by the LH-20 reversed-phase chromatography to get one target substance; Finally, the five kinds of target substances during by separating and purificating carry for color reaction, UV and LC-MS detection, etc. Keywords：Chromatography; chemical constituents; Periplaneta; color reaction