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Õª Òª£º±¾ÎĽ¨Á¢ÁË×ôÆ¥¿Ë¡£¨ZPC£©¶ÔÓ³ÌåµÄ·´Ïà¸ßЧҺÏàÉ«Æײð·Ö·½·¨¡£Ê¹ÓÃChiralcel OD-RH(150 mm¡Á4.6mm£¬5¦Ìm)ÊÖÐÔÖù·ÖÀë×ôÆ¥¿Ë¡¶ÔÓ³Ì壬¿¼²éÁ˲»Í¬Á÷¶¯Ïà×é·Ö¡¢Á÷¶¯ÏàÖÐÒÒë溬Á¿¡¢pHÖµ¡¢ÖùκÍÁ÷ËÙ¶ÔÊÖÐÔ²ð·Ö½á¹ûµÄÓ°Ï졣ȷÁ¢²â¶¨µÄ×î¼ÑÉ«Æ×Ìõ¼þΪ£ºÁ÷¶¯ÏàΪÒÒëæ-Á×Ëá¶þÇâ¼Ø»º³åÒº(pH=6.0£¬V£ºV=35£º65)£»Á÷ËÙ1.0 mL/min£»¼ì²â²¨³¤Îª303nm£»ÖùÎÂ30¡æ¡£½á¹û±íÃ÷×ôÆ¥¿Ë¡ÔÚ0.5-8¦Ìg/mLÖ®¼ä³ÊÁ¼ºÃÏßÐÔ¹Øϵ£¬ÏßÐÔÏà¹ØϵÊýR/S-ZPC£º r£½1£¬Æ½¾ù»ØÊÕÂÊR/S-ZPC·Ö±ðΪ98.23%£¬RSD£½0.48%£¨n£½9£©ºÍ99.19%£¬RSD£½0.52%£¨n£½9£©£¬ÈÜÒºÔÚ24hÄÚÎȶ¨¡£±¾·¨²Ù×÷¼ò±ã¡¢Ò×ÐС¢×¼È·¡¢Öظ´ÐԺá£
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Abstracts: This article develop an RP-HPLC method to separate zopiclone(ZPC) enantiomers. A Chiralcel OD-RH (150mm¡Á4.6mm£¬5¦Ìm) chiral column was used to separate zopiclone enantiomers£®The influence of the different mobile phase£¬the CH3CN content£¬the pH Value£¬the column temperature and the velocity of flow on chiral separation were studied. The best analytic condition was: the mobile phase was composed of acetonitrile and potassium dihydrogen phosphate (pH=6.0, V£ºV=35£º65) and the flow rate was 1.0 mL/min, the detection wavelength was set at 303nm and the column temperature was 30¡æ. The rusults showed that the linear rang of zopiclone was 0.5-8¦Ìg/mL£¬the linear correlation of R/ S-ZPC was1£¬The average recoveries of R/S-ZPC were 98.23% and 99.19%, respectively, with RSD of 0.48% and 0.52% (n=9). The solution was steady within 24h. This method is simple, accurate and with good reproducibility.
Key words: Zopiclone; Separation; High Performance Liquid Chromatography