ÄãºÃ£¬»¶Ó­À´µ½¾­¹ÜÖ®¼Ò [µÇ¼] [×¢²á]

ÉèΪÊ×Ò³ | ¾­¹ÜÖ®¼ÒÊ×Ò³ | Êղر¾Õ¾

¸ßЧҺÏàÉ«Æ×·¨²ð·Ö×ôÆ¥¿Ë¡¶ÔÓ³Ìå_ÖÆÒ©¹¤³ÌÂÛÎÄ·¶ÎÄ

·¢²¼Ê±¼ä£º2015-04-01 À´Ô´£ºÈË´ó¾­¼ÃÂÛ̳
ÖÆÒ©¹¤³ÌÂÛÎÄ·¶ÎÄ Ä¿ ¼ ÖÐÎÄÕªÒª ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¢ñ Ó¢ÎÄÕªÒª ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¢ò Ŀ¼ ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¢ó 1. Ð÷ÂÛ ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­1 1.1Ç°ÑÔ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­1 1.2×ôÆ¥¿Ë¡¼ò½é¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­1 1.3×ôÆ¥¿Ë¡¶ÔÓ³Ìå²ð·ÖÎÄÏ××ÛÊö¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­2 2. ʵÑ鲿·Ö ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­6 2.1 ÒÇÆ÷ºÍÊÔ¼Á ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­6 2.2 ʵÑéÊÔ¼ÁÅäÖÆ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­6 2.2.1 »º³åÈÜÒºÅäÖÆ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­6 2.2.2 Á÷¶¯ÏàÅäÖÆ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­6 2.2.3 ¶ÔÕÕÆ·ÈÜÒºÅäÖÆ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­6 2.2.4 ÑùÆ·ÈÜÒºÅäÖÆ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3 ʵÑé·½·¨¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.1 ²»Í¬É«Æ×Ìõ¼þÏ·ÖÀëÇé¿öµÄ¿¼²ì¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.2 ÏßÐÔÊÔÑé¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.3 ¾«ÃܶÈÊÔÑé¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.4 Öظ´ÐÔÊÔÑé¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.5 »ØÊÕÂÊÊÔÑé¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.6 ÈÜÒºÎȶ¨ÐÔÊÔÑé¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 2.3.7 ÑùÆ·º¬Á¿²â¶¨¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­7 3. ½á¹ûÓëÌÖÂÛ ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­8 3.1 Á÷¶¯Ïà×é³É¶Ô·ÖÀëµÄÓ°Ïì¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­8 3.2 ÒÒëæÌå»ý·ÖÊý¶Ô·ÖÀëµÄÓ°Ïì¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­8 3.3 ÖùζԷÖÀëµÄÓ°Ïì¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­9 3.4 Á÷ËÙ¶Ô·ÖÀëµÄÓ°Ïì¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­9 3.5 Á÷¶¯ÏàpHÖµ¶Ô·ÖÀëµÄÓ°Ïì¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­10 3.6 ×î¼ÑÉ«Æ×Ìõ¼þµÄÈ·Á¢ ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­10 3.7 ÏßÐÔÊÔÑé½á¹û ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­11 3.8 ¾«ÃܶÈÊÔÑé½á¹û ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­12 3.9 Öظ´ÐÔÊÔÑé½á¹û ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­12 3.10 »ØÊÕÂÊÊÔÑé½á¹û¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­13 3.11 ÈÜÒºÎȶ¨ÐÔÊÔÑé½á¹û¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­14 3.12 º¬Á¿²â¶¨½á¹û¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­14 4. ×ܽáÓëÕ¹Íû¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­15 ÖÂл ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­16 ²Î¿¼ÎÄÏ× ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­17 Õª Òª£º±¾ÎĽ¨Á¢ÁË×ôÆ¥¿Ë¡£¨ZPC£©¶ÔÓ³ÌåµÄ·´Ïà¸ßЧҺÏàÉ«Æײð·Ö·½·¨¡£Ê¹ÓÃChiralcel OD-RH(150 mm¡Á4.6mm£¬5¦Ìm)ÊÖÐÔÖù·ÖÀë×ôÆ¥¿Ë¡¶ÔÓ³Ì壬¿¼²éÁ˲»Í¬Á÷¶¯Ïà×é·Ö¡¢Á÷¶¯ÏàÖÐÒÒë溬Á¿¡¢pHÖµ¡¢ÖùκÍÁ÷ËÙ¶ÔÊÖÐÔ²ð·Ö½á¹ûµÄÓ°Ï졣ȷÁ¢²â¶¨µÄ×î¼ÑÉ«Æ×Ìõ¼þΪ£ºÁ÷¶¯ÏàΪÒÒëæ-Á×Ëá¶þÇâ¼Ø»º³åÒº(pH=6.0£¬V£ºV=35£º65)£»Á÷ËÙ1.0 mL/min£»¼ì²â²¨³¤Îª303nm£»ÖùÎÂ30¡æ¡£½á¹û±íÃ÷×ôÆ¥¿Ë¡ÔÚ0.5-8¦Ìg/mLÖ®¼ä³ÊÁ¼ºÃÏßÐÔ¹Øϵ£¬ÏßÐÔÏà¹ØϵÊýR/S-ZPC£º r£½1£¬Æ½¾ù»ØÊÕÂÊR/S-ZPC·Ö±ðΪ98.23%£¬RSD£½0.48%£¨n£½9£©ºÍ99.19%£¬RSD£½0.52%£¨n£½9£©£¬ÈÜÒºÔÚ24hÄÚÎȶ¨¡£±¾·¨²Ù×÷¼ò±ã¡¢Ò×ÐС¢×¼È·¡¢Öظ´ÐԺᣠ¹Ø¼ü´Ê£º×ôÆ¥¿Ë¡£»²ð·Ö£»¸ßЧҺÏàÉ«Æ×·¨ Abstracts: This article develop an RP-HPLC method to separate zopiclone(ZPC) enantiomers. A Chiralcel OD-RH (150mm¡Á4.6mm£¬5¦Ìm) chiral column was used to separate zopiclone enantiomers£®The influence of the different mobile phase£¬the CH3CN content£¬the pH Value£¬the column temperature and the velocity of flow on chiral separation were studied. The best analytic condition was: the mobile phase was composed of acetonitrile and potassium dihydrogen phosphate (pH=6.0, V£ºV=35£º65) and the flow rate was 1.0 mL/min, the detection wavelength was set at 303nm and the column temperature was 30¡æ. The rusults showed that the linear rang of zopiclone was 0.5-8¦Ìg/mL£¬the linear correlation of R/ S-ZPC was1£¬The average recoveries of R/S-ZPC were 98.23% and 99.19%, respectively, with RSD of 0.48% and 0.52% (n=9). The solution was steady within 24h. This method is simple, accurate and with good reproducibility. Key words: Zopiclone; Separation; High Performance Liquid Chromatography
¾­¹ÜÖ®¼Ò¡°Ñ§µÀ»á¡±Ð¡³ÌÐò
  • ɨÂë¼ÓÈë¡°¿¼ÑÐѧϰ±Ê¼ÇȺ¡±
ÍƼöÔĶÁ
¾­¼ÃѧÏà¹ØÎÄÕÂ
±êÇ©ÔÆ
¾­¹ÜÖ®¼Ò¾«²ÊÎÄÕÂÍƼö