提高光养混合生产聚羟基烷酸酯的研究 优化蓄能反应器操作条件的培养

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摘要翻译：
光养混合培养法生产聚羟基烷酸酯（PHAs）是近年来提出的一种生产方法。这些培养物可以在碳的永久存在下进行选择，并且PHA的产生可以在随后的积累步骤中得到提高。为了优化蓄能反应器中PHA的产量，本工作评价了1）初始醋酸浓度，2)光照强度，3)残氮去除对培养性能的影响。结果表明，低醋酸浓度(<30cmm)和20W/gx左右的比光强是获得高聚羟基丁酸酯(PHB)储存产率(0.83+-0.07cmol-PHB/cmol-ACET)和2.21+-0.07cmol-PHB/cmol×D的最佳操作条件。这一速率比以前在非优化积累试验中记录的高三倍，并使PHA含量在不到4h的时间内从15%增加到30%。同时，首次证明了PMC能够利用真正的废物--发酵干酪乳清--生产羟基戊酸盐(HV)含量为12%的PHA。这些结果证实了发酵废物可以作为PMCs生产PHA的底物，并且导致10～20W/gx特定光强的太阳光能级足以驱动光营养PHA的生产过程。
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英文标题：
《Improving polyhydroxyalkanoates production in phototrophic mixed
  cultures by optimizing accumulator reactor operating conditions》
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作者：
Joana Fradinho, Adrian Oehmen and Maria Reis
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最新提交年份：
2019
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分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
  Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30CmM) and specific light intensities around 20W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83+-0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21+-0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in less than 4h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20W/gX are sufficient to drive phototrophic PHA production processes.
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PDF链接：
https://arxiv.org/pdf/1912.00985

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关键词：反应器 Pha Has 蓄能 优化 结果表明 羟基 操作