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摘要翻译：
多重耐药大肠杆菌株引起多种临床感染，已成为全球范围内日益严重的问题。革兰氏阴性菌尤其是大肠杆菌中金属-\b{eta}-内酰胺酶基因的表达非常严重。本研究旨在评估临床分离的大肠杆菌中MBLs的流行情况。从马来西亚细菌感染患者的各种临床样本中共收集到65株大肠杆菌。采用常规微生物学试验对该地区产MBLs的大肠杆菌进行分离鉴定。采用纸片扩散法检测大肠杆菌多药耐药性(MDR)。采用表型和基因型PCR方法检测亚胺培南耐药菌株中金属-\b{eta}-内酰胺酶耐药基因(blaIMP、blaVIM)的存在。65株大肠杆菌中有42株（57.3%）耐多药。来自尿液（19）的分离物产生的MDR(10)明显多于其他来源。19株（29.2%）耐亚胺培南大肠杆菌含有10个MBLs基因，7株（36.8%）含有blaIMP基因，3株（15.7%）含有blaVIM基因。这项研究揭示了临床标本中产生MBL的大肠杆菌分离物的显著发生及其与健康风险因素的联系，表明马来西亚的情况令人担忧。它需要适当的关注，以避免治疗和感染控制管理的失败。
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英文标题：
《Detection of Metallo-\b{eta}-Lactamases-Encoding Genes Among Clinical
Isolates of Escherichia Coli in a Tertiary Care Hospital, Malaysia》
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作者：
Fazlul MKK, Deepthi S, Farzana Y, Najnin A, Rashid Ma, Munira B,
Srikumar C, Nazmul
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最新提交年份：
2019
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分类信息：
一级分类：Quantitative Biology 数量生物学
二级分类：Other Quantitative Biology 其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
The multidrug resistant Escherichia coli strains causes multiple clinical infections and has become a rising problem globally. The metallo-\b{eta}-lactamases encoding genes are very severe in gram-negative bacteria especially E. coli. This study was aimed to evaluate the prevalence of MBLs among the clinical isolates of E. coli. A total of 65 E. coli isolates were collected from various clinical samples of Malaysian patients with bacterial infections. The conventional microbiological test was performed for isolation and identification of E. coli producing MBLs strains in this vicinity. Multidrug Resistance (MDR) of E. coli isolates were assessed using disk diffusion test. Phenotypic methods, as well as genotypic- PCR methods, were performed to detect the presence of metallo-\b{eta}-lactamase resistance genes (blaIMP, blaVIM) in imipenem resistant strains. Out of 65 E. coli isolates, 42 isolates (57.3%) were MDR. The isolates from urine (19) produced significantly more MDR (10) isolates than other sources. Additionally, 19 (29.2%) imipenem resistant E. coli isolates contained 10 MBLs gene, 7(36.8%) isolates contained blaIMP and 3(15.7%) isolates contained blaVIM genes. This study revealed the significant occurrence of MBL producing E. coli isolates in clinical specimens and its association with health risk factors indicating an alarming situation in Malaysia. It demands an appropriate concern to avoid failure of treatments and infection control management.
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PDF链接：
https://arxiv.org/pdf/1904.05198
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