超声辅助下橘核中柠檬苦素提取工艺优化研究
发布时间：2014-10-21
来源：人大经济论坛
目 录
中文摘要I
英文摘要II
目 录III
1 绪论1
1.1 柠檬苦素类似物的含量及分布1
1.2 类柠檬苦素类似物种类和结构1
1.3 柠檬苦素类似物的理化性质2
1.4 柠檬苦素类似物的生物活性2
1.5 柠檬苦素类似物的提取技术4
1.6 柠檬苦素类似物的纯化技术5
1.7 柠檬苦素类似物的分析方法6
1.8 实验思路7
2 实验部分8
2.1 实验原料、试剂与仪器8
2.2 实验步骤9
3 结果与讨论11
3.1 柠檬苦素浓度与吸光度关系的标准曲线11
3.2 单因素实验结果与分析12
3.3 单因素实验结论16
3.4正交结果与分析17
3.5 分离提纯实验结果18
3.6 液相色谱分析19
4 总结与展望20
4.1 总结20
4.2 展望20
致 谢21
参考文献22
摘要：本文首先对料液比、超声时间、超声温度、超声功率、提取剂种类及乙醇浓度等因素对提取的影响进行了考察，确定料液比、超声时间及超声温度为重要影响因素。在此基础上，用正交设计进行提取工艺条件优化，结果表明，在超声辅助下，柠檬苦素提取的最佳工艺条件为：超声温度为30℃，超声时间为30min，料液比为1:70。同时进行了分离提纯研究，经测定柠檬苦素的纯度为84.94%。
关键词：柠檬苦素；超声；提取；工艺优化；正交
Optimization of Ultrasound-assisted Extraction of Limonin in Orange Nuclear by the Orthogonal Design
Abstract: In order to determine the effects of extraction, we first examined the solid-liquid ratio，ultrasonic time，ultrasonic temperature, ultrasonic power, extraction agents types and ethanol concentration in this paper, and finally determined that solid-liquid ratio, ultrasonic time and ultrasonic temperature were the most important factors. On this basis, we optimized extraction process conditions by the orthogonal design. The result showed that the optimum conditions for the extraction conditions under the assistance of the ultrasonic are as follows: ultrasonic temperature of 30℃, ultrasonic time of 30min, solid-liquid ratio of 1:70.At the same time, we also studied the separation purification, and determined the purity of limonin is 84.94%.
Key words: Limonin；Ultrasound；Extraction；Optimization；Orthogonal design
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