毒力因子及β-内酰胺酶编码基因的检测 铜绿假单胞菌的临床分离

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摘要翻译：
背景：铜绿假单胞菌是一种重要的机会性细菌病原体，在全世界范围内引起医院感染，导致治疗失败。本研究旨在比较铜绿假单胞菌毒力特性与b-eta内酰胺酶基因的相关性。方法：从马来西亚吉隆坡色拉洋医院儿科和成人患者中分离铜绿假单胞菌120株。用表型法检测各种毒力因子（磷脂酶、溶血素、明胶酶、DNAse和生物膜）。用双纸片协同试验(DDST)和E-试验检测菌株产超广谱β-内酰胺酶(ESBL)和金属β-内酰胺酶(MBL),用纸片拮抗试验检测AmpCβ-内酰胺酶。结果：共分离铜绿假单胞菌120株（血液、伤口、呼吸道分泌物、粪便、尿液和痰标本各20株）。铜绿假单胞菌的毒力因子分布分别为溶血素(48.33%)、脱氧核糖核酸酶(43.33%)、磷脂酶(40.83%)、明胶酶（31.66%）和生物膜形成（34%）。铜绿假单胞菌中多重b{eta}-内酰胺酶的检出率分别为19.16%的ESBL,7.5%的MBL和10.83%的AmpC。结论：应定期监测，以减少公众伤害
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英文标题：
《Detection of virulence factors and beta-lactamase encoding genes among
  the clinical isolates of Pseudomonas aeruginosa》
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作者：
Fazlul MKK, Najnin A, Farzana Y, Rashid MA, Deepthi S, Srikumar C, SS
  Rashid, Nazmul MHM
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最新提交年份：
2019
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分类信息：

一级分类：Quantitative Biology        数量生物学
二级分类：Other Quantitative Biology        其他定量生物学
分类描述：Work in quantitative biology that does not fit into the other q-bio classifications
不适合其他q-bio分类的定量生物学工作
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英文摘要：
  Background: Pseudomonas aeruginosa has emerged as a significant opportunistic bacterial pathogen that causes nosocomial infections in healthcare settings resulting in treatment failure throughout the world. This study was carried out to compare the relatedness between virulence characteristics and \b{eta}-lactamase encoding genes producing Pseudomonas aeruginosa. Methods: A total of 120 P. aeruginosa isolates were obtained from both paediatric and adult patients of Selayang Hospital, Kuala Lumpur, Malaysia. Phenotypic methods were used to detect various virulence factors (Phospholipase, Hemolysin, Gelatinase, DNAse, and Biofilm). All the isolates were evaluated for production of extended spectrum beta-lactamase (ESBL) as well as metallo \b{eta}-lactamase (MBL) by Double-disk synergy test (DDST) and E-test while AmpC \b{eta}-lactamase production was detected by disk antagonism test. Results: In this study, 120 Pseudomonas aeruginosa isolates (20 each from blood, wounds, respiratory secretions, stools, urine, and sputum samples) were studied. Among Pseudomonas aeruginosa isolates, the distribution of virulence factors was positive for hemolysin (48.33%), DNAse (43.33%), phospholipase (40.83%), gelatinase (31.66%) production and biofilm formation (34%) respectively. The prevalence of multiple \b{eta}-lactamase in P. aeruginosa showed 19.16% ESBL, 7.5% MBL and 10.83% AmpC production respectively. Conclusion: A regular surveillance is required to reduce public healt
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PDF链接：
https://arxiv.org/pdf/1902.02014

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关键词：内酰胺 Quantitative respectively Surveillance distribution 检测 因子 铜绿 isolates 医院