重组DNA与遗传工程
遺傳工程(重組DNA)
重組DNA技術的條件:
1. 基本工具:enzymes2. 基因的來源: purified DNA or cDNA (complementary DNA)3. 傳輸工具:transformation, infection, electroporation, microinjection, gene gun4. 篩選技術: specific antibody screening, hybridization (radioactive isotope or fluorescent agent label),5. 基因表現:expression vectors, protein purification


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