发布时间：2015-04-01 来源：人大经济论坛

制药工程论文范文 目 录 中文摘要 I 英文摘要 II 目录III 1. 绪论1 1.1 前言1 1.2 概述1 1.3 吴茱萸对黄连生物碱溶出率影响的测定方法4 1.4 论文的选题背景及立项依据5 2. 实验部分 6 2.1 黄连中生物碱分析方法的建立6 2.2 吴茱萸中大类组分与黄连生物碱配伍的研究7 2.3 柠檬苦素提取工艺的初步研究 10 2.3.1 材料与仪器 10 3. 结果与讨论11 3.1 黄连中生物碱分析方法的建立 11 3.2 吴茱萸中大类组分与黄连生物碱配伍的研究 12 3.3 柠檬苦素提取工艺的初步研究16 4．总结与展望17 致谢18 参考文献19 摘 要：黄连-吴茱萸药对是寒热配伍的典型方剂，现代临床应用也证明，调整黄连、吴茱萸的用量比例，可用于治疗多种与脾胃功能失调有关的疾病，如胃脘痛、腹痛、呕吐、呃逆、泄泻、便秘、痢疾、反酸嗳气、痞满等。如何控制黄连-吴茱萸药对的配伍比例在中药中是一个值得研究的问题，而在调整配伍比例的过程中出现的一个问题就是黄连小檗碱的溶出率会随着配伍比例的不同而不同。为了研究黄连小檗碱溶出率发生改变的原因及机理，本实验主要包括三个部分：黄连中生物碱分析方法的建立、吴茱萸中大类组分与黄连生物碱配伍的研究以及柠檬苦素提取工艺。结果黄连中小檗碱在Diamonsil-C18（250mm×4.6mm，5μm，迪马公司）色谱柱上，流动相A相为H2O-SDS-H3PO4(1000ml:5g:1ml),CH3CN-H2O-SDS-H3PO4（600mL:400mL:5g:1mL）为B相；采用线形梯度洗脱程序：0min，25%B；15min，50%B；20min，80%B；50min，80%B；60min，90%B；流速0.8mL/min；柱温40℃；检测波长360nm的分析条件下分离度、重现性、精密度和加样回收率均较理想。 在吴茱萸大类提取物组分和黄连小檗碱的配伍过程中，黄连小檗碱的溶出率都有一定降低，但以和乙酸乙酯提取物配伍后降低最多。最后提取出的柠檬苦素0.69g。 关键词：吴茱萸、黄连、提取、小檗碱、溶出率 Abstract：Huanglian - rutaecarpine-right yes compatibility of typical prescription cold and heat, modern clinical applications show that berberine adjustment, the amount of rutaecarpine ratio can be used to treat a variety of functions of the spleen and stomach disorders and related diseases, such as Epigastralgia, abdominal pain, vomiting, hiccup, Diarrhea, constipation, diarrhea, anti-acid Aiqi, such as Seoul Man . How to control Huanglian - rutaecarpine drugs on the compatibility of traditional Chinese medicine is a study of the problem, and in the adjustment of the ratio of compatibility in the process of Huanglian is a question of the dissolution rate of berberine as the compatibility of the different and different. In order to study the dissolution rate of Huanglian berberine change and the reasons for the mechanism of this experiment consists of three main parts: Huanglian alkaloids in the establishment of analytical methods, like the Chinese University of rutaecarpine components and compatibility of Huanglian alkaloids and bitter-lemon Extraction Process. The results of berberine in Huanglian Diamonsil-C18 (250mm × 4.6mm, 5 μ m, Dumas) column, the mobile phase A phase of H2O-SDS-H3PO4 (1000mL: 5g: 1mL), B phase of CH3CN-H2O - SDS-H3PO4 (600mL: 400mL: 5g: 1mL); using linear gradient elution procedures: 0 min, 25% B; 15min, 50% B; 20min, 80% B; 50min, 80% B; 60min, 90% B. Flow rate of 0.8 mL / min; column temperature of 40℃; detection wavelength of 360 nm under the conditions of separation, reproducibility, precision and processing recovery than ideal. In extracts of rutaecarpine largest category of components and compatibility Huanglian berberine in the course of the dissolution rate of Huanglian Berberine has certainly reduced, but to extract and ethyl acetate after the lower maximum compatibility. Finally extracted from the plight of 0.69 g lemon. Keywords：Rutaecarpine, Huanglian, Extraction, berberine, the dissolution rate