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¡²5¡³ ¼Ö´æÓ¢.ȱпÓ벹п£ÛJ£Ý.΢Á¿ÔªËØÓ뽡¿µÑо¿,2001,18(1):77.
¡²6¡³ Fengyuan Piao,Kazuhit Yokoyama,Ning Ma,et al.Subacute toxic effects of zinc on various tissues and organs of rats£ÛJ£Ý.Toxicology Letters,2003,145:28-35.
¡²7¡³ ÁººèÑã,¸ßºêΰ,ÇúÓÀÀû,µÈ.пÓëάÉúËØA»¥×÷ЧӦµÄÑо¿£ÛJ£Ý.ËÄ´¨ÐóÄÁÊÞÒ½,2003,30(5):38-39.
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