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ʳƷ¿ÆѧÓ빤³ÌÂÛÎÄ·¶ÎÄ ¢ò Ŀ¼ ÖÐÎÄÕªÒª¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­. ¢ñ Ó¢ÎÄÕªÒª¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­...¢ò Ŀ¼¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¢ó 1.Ð÷ÂÛ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..1 2.ʵÑ鲿·Ö 2 2.1²ÄÁϺͷ½·¨¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..2 2.1.1²ÄÁÏ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.2 2.1.2·½·¨¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.2 3.½á¹ûÓëÌÖÂÛ.................................................5 3.1½á¹û...................................................5 3.1.1¾úÖê¹Û²ì..........................................5 3.1.2¾úÖê·¢½Í..........................................6 3.1.3¿¹¾úµ°°×µÄ·ÖÀë´¿»¯................................6 3.1.4¿¹¾úµ°°×Àí»¯ÐÔÖʵÄÑо¿¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..7 3.2ÌÖÂÛ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­9 3.2.1·¢½Í¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­..10 3.2.2¿¹¾úµ°°×µÄ·ÖÀë´¿»¯ 4.×ܽáÓëÕ¹Íû¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.6 4.1×ܽᡭ¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­¡­.6 4.2Õ¹Íû......................................................6 ÖÂл...........................................................8 ²Î¿¼ÎÄÏ×.......................................................9 ÕªÒª£º¿Ý²ÝÑ¿æ߸˾ú¶ÔºÚÇúùÓÐÇ¿ÁÒµÄÒÖÖÆ×÷Óá£ÓÚÅàÑøÒºÉÏÇåÖмÓÈë60%±¥ºÍ¶ÈµÄÁòËá麟Áµí¿ÉµÃµ°°×´ÖÌáÒº¡£¸Ãµ°°×´ÖÌáÒº¾­Í¸ÎöÍÑÑκóµÃµ½³õ²½´¿»¯¡£¸Ãµ°°×Ä͸ßΣ»ËüÔÚ´Ó4µ½10µÄºÜ¹ãµÄpH·¶Î§ÄÚÄܱ£³Ö»îÐÔ£¬µ«¶Ô¸ßpHÖµÃô¸Ð£»¶Ô×ÏÍâÏß²»Ãô¸Ð¡£ ¹Ø¼ü´Ê£º¿Ý²ÝÑ¿æ߸˾ú£»¿¹¾úµ°°×£»·ÖÀë´¿»¯£»¿¹¾úÌØÐÔ Abstract:Bacillus subtilis showed strongly antibacterial activity against Aspergillus niger.A crude antibacterial preparation(CAP) was obtained by adding ammonium sulphate to supernatant fluid of B.subtilis to a finial saturation of 60%.CAP was purified by dialysision.CAP is thermostable;its active pH range is wide,from 4.0 to more than 10.0,but it is sensitive to high pH;it is sensitive to UV rays. Key words:B.subtilis antibacterial peptite separation and purification antibacterial character