农杆菌介导转录因子CBF转化甘蓝型油菜_生物工程专业论文-经管之家官网！

扫码加入经济学习群

目 录
中文摘要I
英文摘要II
目录III
1. 绪论1
1.1 冷驯化及CBF家族概述1
1.2 CBF家族与植物抗冻性3
2. 材料与方法7
2.1 材料7
2.2 方法8
3. 结果与分析12
3.1 转基因油菜植株的获得12
3.2 转化植株PCR检测13
3.3 转化植株Southern杂交分析13
4. 总结与展望15
致谢16
参考文献17

摘 要：油菜是我国最主要的油料作物，有甘蓝型油菜、白菜型油菜和芥菜型油菜等9个种，其中许多是具有重要经济价值的油料与蔬菜作物。但是当环境温度低于10℃～20℃时油菜就会受到伤害，影响它的正常生长发育，严重的甚至造成死亡。目前很多作物在不同程度上都受到低温的影响，低温这一重要的环境因子正严重影响油菜的生长、发展、生存及分布。因此，针对耐逆性（抗寒性）相关的转基因油菜的研究，本试验以成熟油菜“超油2号”种子培养出的下胚轴愈伤组织为受体材料，运用农杆菌介导法将转录因子CBF基因导入供试品种的基因组中，得到的油菜植株经PCR检测，Southern杂交验证分析，获得转基因植株60株，转化频率为1.2%。已转入目的基因植株的耐逆性的鉴定正在准备之中。
关键词：CBF；根瘤农杆菌；PCR；Southern杂交

Agrobacterium tumefaciens-Mediated Transformation of Brassica napus L. with CBF Gene
Abstract: Rape is the most important oilseed crop, there are 9 kinds of rapes, Brassica napus, he cabbage rape, the leaf mustard rape and so on. Many of them have the important economic values. But when the environment temperature is lower than 10℃~ 20℃ rape may be injured, its normal growth would be affected, even worse, it would be died. At present, many crops affected by low temperature in the varying degree. But the low temperature of this important environmental factor that is seriously affecting rape's growth, development, survival and distribution. In this study, a gene for CBF was incorporated into genome of "super-oil No.2", a new Brassica napus L. strain with super-high oil content, by Agrobacterium tumefaciens-mediated transformation with hypocotyl segments as recipient materials. Based on PCR assay and Southern hybridization analysis, 60 transgenic plants were obtained and the transformation frequency was 1.2%. The identification of transgenic plants is being prepared.
Keywords : CBF; Agrobacterium tumefaciens ; PCR; Southern hybridization

「经管之家」APP：经管人学习、答疑、交友，就上经管之家！
免流量费下载资料----在经管之家app可以下载论坛上的所有资源，并且不额外收取下载高峰期的论坛币。
涵盖所有经管领域的优秀内容----覆盖经济、管理、金融投资、计量统计、数据分析、国贸、财会等专业的学习宝库，各类资料应有尽有。
来自五湖四海的经管达人----已经有上千万的经管人来到这里，你可以找到任何学科方向、有共同话题的朋友。
经管之家（原人大经济论坛），跨越高校的围墙，带你走进经管知识的新世界。
扫描下方二维码下载并注册APP