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液相色谱串联质谱法测水产品中的硝基呋喃代谢物_食品科学与工程论文

发布时间:2015-04-01 来源:人大经济论坛
食品科学与工程论文范文 中文摘要……………………………………………………………………………..1 英文摘要……………………………………………………………………………..1 1 绪论……………………………………………………………………………2 1.1 前言……………………………………………………………………...2 2 试验部分……………………………………………………………………….4 2.1试剂和材料………………………………………….……………..… ..4 2.2仪器和设备………………………………………………………………. 6 2.3仪器参数与测定 ……………………………………………………… 6 3 实验方法……………………………………………………………………….7 3.1试样制备………………………………………………………………….7 3.2分析步骤…………………………………………………………………7 3.3净化步骤 ………………………………………………………………..8 3.4校正曲线………………………………………………………………….8 结果与分析…………………………………………………………..……….8 4.1过柱量选择………………………………………………………………..8 4.2衍生温度和时间的选择……………………….………………………..10 4.3提取净化方法的选择…………………………….……………………..10 硝基呋喃代谢物衍生物的工作曲线及相关系数…………………………..11 注意事项……………………………………………………………………..14 回收率和精密度……………………………………………………………..14 参考文献……………………………………………………………………..16 致谢…………………………………………………………………………..17 附录A:标准品色谱图 摘要:由于硝基呋喃类药物对人类健康的潜在危害,欧盟已经禁止其在动物食品生产中使用。采用固相萃取(SPE)前处理净化技术, 高效液相色谱-串联质谱(LC-MS/MS)法测定水产中呋喃它酮(furaltadon)、呋喃西林(furacilin)、呋喃妥因(titrofurantoine)、呋喃唑酮(furazolidonum)代谢产物的残留量。分析物采用多反应监测方法(MRM),同位素内标法定量。 关键词:硝基呋喃代谢物;水产品;固相萃取;高效液相色谱-串联质谱 Highly effective liquid chromatography - series mass spectrography determination pesticide residues nitryl fu nan kind of antibiotic metabolite Abstract:The use of nitrofurans in food-producing animals has been banned in EU, because they may represent a public health risk. A method has been developed to determine the pesticide residues of furaltadon, furacilin, titrofurantoine and furazolidonum in Aquatic products by LC-MS/MS coupled with the solid phase extraction(SPE).Identification was achieved by using multiple reaction monitoring(MRM). The quantification was performed with isotope as the internal standard. Key word:nitrofuran metablolites;pesticide residues;SPE;LC-MS/MS
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