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目 录
中文摘要I
英文摘要II
目录III
1. 绪论1
1.1 X7的性质1
1.2 X7的功用2
1.3 X7的开发3
2. 实验部分5
2.1 实验器材5
2.2 实验方法6
3. 结果与讨论8
3.1 种子配方及种龄种量实验8
3.2 pH、P、Ca实验 9
3.3 C源调整实验11
3.4 N源调整实验14
3.5 无机盐实验16

4. 总结与展望19
致谢20
参考文献21

摘　要：X7是一种具有极强的抗氧化性的酮式类胡萝卜素 ,在医药 ,食品 ,化妆品等方面有着极广阔的应用前景。目前,生物技术生产X7的两种主要方法:藻类培养法和酵母发酵法。
A酵母是X7生产中最具潜力的生物来源。根据A酵母发酵工艺原理, 本实验对A酵母菌株产生X7的种子培养条件和发酵培养条件进行了初步研究, 并主要对发酵过程作了动态分析. 结果表明: 种子培养基组成、发酵培养基组成以及初始pH值、摇瓶装量与培养时间和温度等, 均对该菌细胞的X7产量有影响。
由此，对A酵母发酵生产X7的种子培养和发酵条件包括：种子配方，种龄种量，发酵配方等进行优化,得到最佳培养条件。在此条件下发酵24 h和28 h ,得到比优化前高几倍的X7效价。达到优化工艺条件、提高生产效率的目的。
关键词： X7； A酵母； 发酵； 优化

The handicraft studies of the zymotechnics producing of X7

Abstract: X7 is a kind keto form carotenoid which has strong inoxidability. It has the extremely broad application in the medicine, food, and cosmetics. At present, there are two main methods to produce X7: algal culture and yeast fermentation.
The A yeast is the most potential biological origin in the X7 production. According to the craft principle of A yeast fermentation, the experiment has carried out a pilot study. And the study reffers to the condition in A yeast culture and mainly in A yeast fermentation. The results indicated that, all of the following has an effect on X7 production. They are: the culture medium of culture and fermentation, initial pH, filling level, as well as raise time and temperature.
From this, the experiment has been carried on to optimize the condition in A yeast culture and fermentation. It concludes the culture medium of culture and fermentation, the vaccination quantity, raise time, raise quantity, initial pH and so on. Then it obtains the best culture condition. By fermenting for 24 h and 28 h under this condition, the yeast obtains a several times bigger yield of X7, which is compared to before the optimization. So the goal of the optimization of process conditions, and the enhance of the yield is achieved.
Key words: X7; A yeast; Fermentation; Optimization

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