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八角茴香中莽草酸的提取与纯化_制药工程论文范文
制药工程论文范文 目 录 中文摘要…………………………………………………………………………………… Ⅰ 英文文摘…………………………………………………………………………………… Ⅱ 目录………………………………………………………………………………………… Ⅲ 1 绪论………………………………………………………………………………………1 1.1 八角茴香概述……………………………………………………………………1 1.2 莽草酸的概述……………………………………………………………………2 1.3 存在的问题以及本研究的意义…………………………………………………3 1.4 研究的内容………………………………………………………………………4 1.5 预期的结果………………………………………………………………………4 2 实验部分…………………………………………………………………………………5 2.1 莽草酸的提取条件的研究………………………………………………………5 2.2 莽草酸的纯化工艺………………………………………………………………6 2.3 莽草酸检测方法的建立…………………………………………………………7 3 结果与讨论………………………………………………………………………………8 3.1 提取结果与讨论…………………………………………………………………8 3.2 纯化结果与讨论…………………………………………………………………9 4 总结与展望………………………………………………………………………………12 致谢 …………………………………………………………………………………………13 参考文献 ……………………………………………………………………………………14 附录 …………………………………………………………………………………………15 摘要：莽草酸，针状结晶，有抗炎、镇痛作用，是抗癌药物中间体。主要存在于八角属植物中，而以八角茴香中的含量最高。工艺落后导致提取收率低成本高，无法满足扩大再生产的要求。此课题将研究八角茴香中提取莽草酸的工艺，较为系统的对八角茴香果实——莽草酸提取条件的因素：提取溶剂类型、溶剂浓度、提取次数、料液比，提取时间等进行考察，在此基础上应用正交实验确定出最佳提取工艺路线。并且通过多次重结晶得到高纯度的莽草酸晶体。兼从降低成本、资源整合利用、对环境友好等角度使工艺达到最优化、经济效益和社会效益最大化，非常具有实际意义。 关键词：八角茴香 ；莽草酸 ；提取； Abstract: Shikimic acid, a crystal powder, has the effect of scorching, easing pain and so on, is the anticancer medicine’s Intermediates. It mainly exist in Iuicium verum, especially in Illicium Verum Hook.f., . Now the backward process led to high waste and cost, the factory has no way to satisfy the marcket’s request. In this paper, we will study the extraction of the shikimic acid from illicium verum Hook. f., contains extraction solvent, extraction time and so on. It is a meaningful experiment. Key Words: Illicium Verum Hook.f. ; Shikimic Acid; Extract；
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甘氨酸与亚铁离子配位反应的动力学研究_制药工程论文范文
制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 引言2 1.2 甘氨酸亚铁配合物的分子结构4 1.3 甘氨酸的制备方法4 2. 实验部分6 2.1 实验仪器和材料6 2.2 实验装置和实验方程6 2.3 典型的实验过程 8 3. 结果与讨论8 3.1 配合物的吸收光谱 8 3.2 配合物的组成9 3.3 甘氨酸与亚铁离子配位反应的级数 11 3.4 温度对甘氨酸与亚铁离子配位反应的影响13 3.5 离子强度对甘氨酸与亚铁离子配位反应的影响 15 3.6 pH值对甘氨酸与亚铁离子配位反应的影响 17 3.7 讨论 19 4．小结20 5．总结与展望21 致谢22 参考文献23 摘要：本论文用紫外-可见光谱法研究甘氨酸与亚铁离子配位反应．考察了温度、离子强度、配位比及pH值等因素对配位反应的影响。结果表明：当I=0.02；甘氨酸与亚铁离子的配位比为4：1；pH=5.0时，甘氨酸亚铁有最大的反应速率。在此条件，根据Arrhenius方程和Absolute-rate theory方程求得其活化能为4291.35J/mol，自由能△G≠为1786.60J/mol。而温度对配位反应的影响表现不是很突出。在甘氨酸与亚铁离子分别大量过量的条件下得出配位反应是二级反应。最后本文提出了可能的配位反应机理，为制备高纯甘氨酸亚铁提供理论依据。 关键词：甘氨酸亚铁 配位反应 紫外可见光谱法 动力学 Abstract: In this dissertation，the complexation between glycine and ferrous ions was studied by UV - Vis spectroscopy .The influences of complexation, such as temperature, ionic strength, coordination and pH value were observed. The result shows that: the ionic strength I = 0.02;the complex rate of glycine and ferrous ions (Gly / Fe) is 4:1; pH = 5.0，the rate constant of the complexation had the largest separately．It wsa accounted that the Activation energy Ea was 4291.35J/mol by the equation of Arrhenius, and free energy △G≠ was 1786.60J/mol by the equation of Absolute-rate theory，and the temperature was not affect the the formation rate obviously. the complexation between glycine and ferrous ions was second-order reaction when glycine excess and ferrous ions excess. In the end，The theoretical basis was provided for the preparation of high purity ferrous glycine. Key words: iron(Ⅱ)-glycine UV-visible spectroscopy Coordination reaction dynamics
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壳聚糖-羧甲基纤维素温敏凝胶的研制及其性能研究_制药工程论文
制药工程论文范文 目 录 中文摘要 I 英文摘要 II 目录 1 绪论 1 1.1 凝胶 1 1.2 高分子可降解材料 1 1.3 研究内容 3 2 实验部分 4 2.1 实验试剂和仪器 4 2.1.1 实验试剂 4 2.1.2 实验仪器 4 2.2 实验方法 5 3 结果与讨论 9 3.1 原料的检测 9 3.2 CS-CMC温敏凝胶的形成 12 3.3 凝胶制备条件对凝胶形成的影响 13 3.4 CS-CMC凝胶的处方优化 15 3.5 凝胶性能测试 16 4 总结与展望 20 致谢 21 参考文献 22 摘要：本论文研究制备了能够在生理条件下发生相转化的壳聚糖-羧甲基纤维素凝胶。首先用酸度计法测得羧甲基纤维素钠的取代度、双突越滴定法测得壳聚糖的脱乙酰度，用乌氏粘度计测得羧甲基纤维素钠、壳聚糖的粘均分子量，然后通过物理共混技术制备得到互穿网络的壳聚糖-羧甲基纤维素水凝胶。通过对水凝胶制备条件的考察，优选出具有快速响应性的水凝胶，并对制得的凝胶进行了确证和性能测定，如红外光谱、溶胀度、透光率、相转变温度等。实验结果表明：当壳聚糖溶液pH=4.6，质量分数为1% 羧甲基纤维素钠溶液与等浓度壳聚糖溶液以0.8的体积比混合时，壳聚糖-羧甲基纤维素溶液在 41℃发生凝胶化，凝胶时间为1min。该凝胶在室温或低于室温时可保持液态，当温度达到体温时可快速发生凝胶化，是一种可供医疗使用的智能水凝胶。 关键词：壳聚糖；羧甲基纤维素钠；温敏性 Abstract: This thesis is to introduce the preparation of the chitosan-carboxymethyl cellulose (CS-CMC) hydrogel, which can occur the pHase transformation under pHysiological conditions. Firstly, the substitution degree of sodium carboxymethyl cellulose was measured by pH meter titration method, and the deacetylation degree of chitosan was measured by two abrupt titration method. The viscosity average molecular weights of sodium carboxymethyl cellulose and chitosan were determined by ubbelohde viscometer. And then the interpenetrating network hydrogel of chitosan-carboxymethyl cellulose (CS-CMC) was prepared by pHysical blending technology. The fast response hydrogel was obtained after the preparation condition study. Finally the characteristics identification and performance measurement of the obtained gel were carried out, such as infrared spectroscopy, swelling ratio, transmittance, pHase transition temperature, and so on. The results show that when the pH of CS solution reaches 4.6, 1% CMC solution mixed with equal concentrations of CS solution in the volume ratio of 0.8, the solution gelled at 41 ℃, the gel time was less than 1min. This hydrogel can be kept in the liquid at room temperature or below room temperature, while when the temperature reaches body temperature, it can quickly gelled, This hydrogel is smart and can be used in medical treatment. Key words: Chitosan; Carboxymethyl Cellulose; thermo-sensitive
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二氟溴乙酸乙酯的制备_制药工程论文范文
制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 导言1 1.2 二氟溴乙酸乙酯制备技术进展1 2. 实验部分7 2.1 实验仪器与试剂7 2.2 实验操作步骤8 3. 结果与讨论12 3.1脱氯化氢与溴化工艺的研究12 3.2 氧化与酯化工艺的研究14 3.3.精馏16 3.4 本章小结19 4. 总结与展望20 致谢21 参考文献22 摘要：本文讨论了二氟溴乙酸化合物的制备技术,并且具体对合成溴二氟乙酸乙酯的工艺路线进行探讨，从收率角度以及三废回收，工业化生产等几个重要因素考虑。综合进行比较分析，选择以1，1-二氟-1，2，2-三氯乙烷（F122）为原料，先脱氯化氢，再溴化，然后酯化合成，采用GC1690气相色谱进行分析，检验。与对照品对照，确定产品及产品的纯度。研究结果表明：在脱氯化氢溴化反应中，当达到1.3，反应温度达到50℃，滴加时间达到60分钟，加溴反应温度达到55℃时，最佳收率为84％。在氧化和酯化反应中，当F123与发烟硫酸的摩尔比达到0.56，F123与发烟硫酸的反应温度达到70℃， F123与发烟硫酸的酯化反应温度达到35℃时，最佳收率为80％。此路线的最佳收率为67%。 关键字： 二氟溴乙酸乙酯； 溴化 ；1，1-二氟-1，2，2-三氯乙烷 Abstract: This paper discussed the synthesis of ethyl bromodifluoroacetate and further explored the process of ethyl bromodifluoroacetate. Considering the factors such as profit, wastes’ recycling and industrial production, 1，1-difluoro -1，2，2- trichloroethane was chosen as the raw material. The process followed as below: first, dehydrochlorination, second, bromination, and third esterification. The purity of the product was analyzed and detected by GC1690. The optimal reaction conditions as following: ethanol as solvent, 1.3 equivalent of sodium hydroxide for reaction, condensation reaction temperature was 50℃, the temperature of esterification was 35℃, the best yield of ethyl bromodifluoroacetate was 67%. Key words: Ethyl bromodifluoroacetate; bromination; 1，1-difluoro -1，2，2- trichloroethane
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salen催化氧化环己烷制备环己酮_制药工程论文范文
制药工程论文范文 目 录 中文摘要I 英文摘要.......II 目录...................................................................III 1. 绪论1 1.1 前言1 1.2 研究现状1 1.3 本文研究的内容...................................................4 2. 实验部分 ............................................................5 2.1 实验仪器与试剂 .................................................5 2.2 实验操作步骤 ...................................................6 3. 结果与讨论.......................................................... 12 3.1 不同催化剂的催化效果............................................ 12 3.2 [Co(Ⅱ)（salen）]和 [Fe(Ⅲ)（salen）Cl]在不同溶剂中的催化效果.....12 3.3 反应温度对催化效果的影响........................................ 13 3.4 不同氧化剂对催化效果的影响...................................... 13 3.5不同反应时间对催化效果的影响.....................................14 3.6催化机理........................................................ 14 3.7小结............................................................ 15 4. 总结和展望.......................................................... 16 4.1 总结............................................................ 16 4.2 展望............................................................ 16 致谢................................................................... 17 参考文献............................................................... 18 摘要：本文制备了[Fe(Ⅲ)（salen）Cl]、[Mn(Ⅲ)（salen）Cl]和[Co(Ⅱ)（salen）]三种催化剂，并将其应用于环己烷催化氧化的工艺中。催化剂采用傅立叶红外光谱进行表征。实验以H2O2和过氧化氢叔丁基为氧化剂，乙腈或丙酮作为溶剂，在60℃的温度下反应。实验发现以丙酮为溶剂，[Co(Ⅱ)（salen）]为催化剂的过氧化氢体系中反应6小时，环己烷的转化率高达62.5%。探讨了环己烷催化氧化下的反应机理。 关键词： salen催化剂;环己烷;仿生催化；催化氧化；反应机理 Abstract: Salen complexes of iron(Ⅲ)、manganese(Ⅲ) and cobalt(Ⅱ) were synthesized and employed as catalysts in the oxidation of cyclohexane. The catalysts were characterized by Fourier Transform Infrared Spectrometer. The reactions were carried out in acetonitrile or acetone at 60℃，using hydrogen peroxide and tert-butylhydroperoxide as oxidation. Acetone as soluat，cyclohexane conversions up to 62.5% were obtained for the system [Co(Ⅱ)（salen）]/ H2O2 . A possible mechanism was proposed for the reaction by Oxidation of cyclohexane. Keywords: salen catalyst；cyclohexane ；biomimetic catalyst；oxidation；kinetic resolution
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5-氨基-3-巯基-1, 2, 4-三唑的合成_制药工程论文范文
制药工程论文范文 目 录 中文摘要 I 英文摘要 II 目录 III 1 绪论 1 1.1 除草剂的研究现状及其分类 1 2 实验部分 9 2.1 实验试剂9 2.2 实验仪器9 2.3 实验装置10 2.4 实验步骤10 3 结果与讨论..12 3.1 以氨基胍碳酸氢盐与硫氰酸铵为原料合成AMZ12 3.2 以氨基胍（盐）与二硫化碳为原料合成AMZ13 3.3 以肼与硫氰酸铵为原料合成AMZ14 3.4 合成AMZ的最佳工艺路线14 4 总结与展望17 致谢18 参考文献19 摘 要：5-氨基-3-巯基-1，2，4-三唑（简称AMZ）是重要的有机合成中间体，在农药、医药和成像材料等领域有广泛的用途。本文在查阅大量文献的基础上，通过比较各种方法的优缺点提出了合成5-氨基－3－巯基－1，2，4－三唑（简称AMZ）最理想的路线：以氨基胍碳酸氢盐与硫氰酸铵为原料合成目标产物。并通过对反应物物质的量之比、反应溶剂量以及反应的温度的实验探索，得出合成目标产物的最佳工艺条件是：氨基胍盐与硫氰酸铵的物质的量之比为1：1.2；氨基胍盐与氢氧化钠物质的量之比为1:1.3；加盐酸温度为98℃；加氢氧化钠温度110℃；在上述优化条件下，AMZ的产率为83.90%。 关键词：农药；除草剂；合成；5-氨基-3-巯基-1,2,4-三唑; Abstact: 5-amino-3-mercapto-1,2,4-triazole （AMZ） is an important intermediate in organic synthesis. It has widespread usage in the fields of pesticide, medicine, imaging materials and so on. Based on the consultation of a large number of literature and the comparison of the advantages and disadvantages of various methods about the synthesis of 5-amino-3-mercapto-1,2,4-triazole （AMZ）, the best route is: take aminoguanidine bicarbonate and ammonium thiocyanate as raw materials to synthesize the product. And based on the experimental explorations of the amount of adducts, reaction solvent volume, and reaction temperature, the optimum conditions of this route are: the ratio of the amount between aminoguanidine salt and ammonium thiocyanate is 1:1.2; the ratio of the amount between aminoguanidine salt and sodium hydroxide is 1:1.3; the temperature of hydrochloride is 98 ° C; the temperature of sodium hydroxide is 110 ° C; In optimum conditions, the yield of AMZ is 83.90%. Key words: pesticide; herbicide; synthesis; AMZ;
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YBGY胶囊的定量分析方法的建立_制药工程论文范文
制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 概述1 1.2 中药及其制剂分析的一般程序1 1.3 YBGY胶囊的定量分析方法的建立6 2. 实验部分7 2.1 仪器与试剂7 2.2 紫外实验7 2.3 HPLC实验7 3. 结果与讨论10 3.1 紫外的结果与讨论10 3.2 高效液相色谱的结果与讨论10 4．总结与展望18 致谢19 参考文献20 摘 要：药物是一类特殊的商品，尤其是中药制剂，成分复杂，而且大多数中药的有效成分尚不十分清楚，其质量的优劣，不但直接影响预防和治疗疾病的效果，而且密切关系到人民的健康与生命安全。因此，药物质量的研究对于保障药物的安全性和有效性具有重要的意义。本论文通过对YBGY胶囊的质量标准进行了研究，建立了定量分析方法。采用反相高效液相色谱法对其君药YC中的两个有效成分进行了定量分析，流动相为0.025mol/L H3PO4(用三乙胺调节pH为3.0)-甲醇-乙腈（75:26:15），检测波长为345nm,流速：1.0ml/min，柱温：30℃，结果表明6,7-二甲氧基香豆素的线性范围为1.3-13μg/ml，回收率为96.62%；6-羟基-7-甲氧基香豆素的线性范围为2.36-23.6μg/ml，回收率为96.50%，分析方法简便、灵敏度高、重现性好，为评价YBGY胶囊质量提供了定量依据。 关键词：YBGY胶囊；高效液相色谱法；定量分析 Abstract: Medicinal drug is a special kind of commodity, especially for chinese materia medica preparation , complex sentence , and most effective elements of traditional chinese medicine is not very clear ,its quality directly connected to people’s health and life. Therefore, it is of great significance to research on the qualification control of drug to ensure its safety and effectiveness. We have developed a quantitative method. HPLC was applied to determinate the two effective components in the chief herb-YC, with the liquid phase 0.025mol/L H3PO4(pH was adjusted to 3.0 with triethylamine)-methol-acetonitrile; the detection wavelength:345nm, the rate:1.0 ml/min and the column temperature:30℃. Results showed that the linear range of 6,7-dimedthoxycoumarin is 1.3-13ug/ml with the recovery of 96.62%; the linear range of 6-hydroxy-7-methoxycoumarin is 2.36-23.6ug/ml with the recovery of 96.50%. The analytical method is simple, sensitive and high reproducibility, thus provide qualitative and quantitative proof for the quality control of Capsule YBGY. Keywords：YBGBcapsule; High Performance Liquid Chromatography(HPLC); quantitative analysis
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白花前胡中花椒毒素的超声水提工艺研究_制药工程论文
制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 草本的历史沿革3 1.2 白花前胡的性质3 1.3 化学成分的研究3 1.4 花椒毒素的临床应用4 1.5 常用提取法6 2. 实验部分8 2.1 仪器与材料8 2.2 原理与方法8 2.3 检测波长的选择92.4 各因素对吸光度的影响10 2.4.1超声温度对吸光值的影响10 2.5 确定花椒毒素最佳提取工艺条件的正交试验12 3. 结果与分析12 3.1 超声温度对吸光值的影响结果12 3.2 料液比对吸光值的影响结果13 3.3 超声功率对吸光值的影响结果13 3.4 超声时间对吸光值的影响结果14 3.5 正交试验结果15 3.6 最佳试验结果的验证16 4．结论与展望16 4.1 结论17 4.2 存在的问题18 4.3 展望18 致谢19 参考文献20 附录22 摘 要:本论文主要研究在超声辅助下的水提白花前胡中,花椒毒素的最佳提取工艺。方法：以白花前胡中花椒毒素提取量的吸光值为指标，在单因素试验的基础上，采用正交试验设计法对白花前胡的超声辅助水提工艺进行优选，考察超声提取温度、超声提取功率、超声提取时间和料液比对花椒毒素提取量吸光值的影响。结果：超声辅助水提白花前胡中花椒毒素的最佳工艺为：超声温度为50℃，超声功率为176W，超声提取时间为40min，料液比为1：20。结论：所确定的最佳提取工艺可合理提取白花前胡中的活性成分。 关键词：白花前胡；花椒毒素；8-甲氧基补骨脂；超声；水提 Abstract: This paper mainly studies in the ultrasound-assisted water extraction process of Xanthotoxin from Peucedanum praeruptorum Dumn.With the absorbency of Peucedanum praeruptorum Dumn for index,to xanthotoxin amount of a pigment absorbance value of the target in single-factor test, based on the orthogonal design method of ultrasonic assisted xanthotoxin water extraction process was optimized to investigate ultrasonic extraction temperature, extraction power, extraction time and solid to liquid ratio on the extraction of xanthotoxin component of absorbency. The ultrasound-assisted water extraction and xanthotoxin in a prime optimum technology for: ultrasonic temperature is 50 ℃, ultrasonic power is 176W, extraction time of 40min, solid to liquid ratio of 1:20. The optimized method can be applied for the extraction of active components in roots of Peucedanum praeruptorum Dunn． Keywords：Peucedanum praeruptorum Dumn；xanthotoxin；8-methoxypsoralen；ultrasound-assisted water extraction
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美洛昔康的含量测定和稳定性考察_制药工程论文范文
制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1. 绪论1 1.1 概述1 1.2 紫外可见光谱2 1.3高效液相色谱法4 2. 实验部分8 2.1 仪器与试剂8 2.2 紫外实验8 2.3 HPLC实验9 3. 结果与讨论11 3.1 紫外的结果与讨论 11 3.2 高效液相色谱的结果与讨论 13 4．总结与展望18 致谢20 参考文献21 摘要：美洛昔康是一种非甾体抗炎药，有很强的抗炎镇痛作用。临床研究表明美洛昔康可有效地治疗风湿关节炎和骨关节炎，相对于传统的抗炎药更加安全、长效，且副作用更低。本论文用紫外分光光度法来测定制剂中美洛昔康的含量，用高效液相色谱法来考察美洛昔康的稳定性并测定美洛昔康的有关物质及降解产物的含量。结果表明，美洛昔康的最大吸收波长为372nm，美洛昔康在0.580～0.869μg/ml浓度范围内线性关系良好，可得回归方程为：Y=0.4785X+0.0138，R=0.9996；美洛昔康的有关物质及降解产物的测定采用反相高效液相色谱法，流动相选择为0.05mol/L磷酸二氢铵(pH4.5)—甲醇(47:53)，检测波长370nm，结果表明，美洛昔康对酸、氧化剂、光不稳定，对碱和高温较稳定。 关键字：紫外—可见分光度法；高效液相色谱法；美洛昔康 Abstract: Meloxicam is a non-steroidal anti-inflammatory drugs. It has a strong anti-inflammatory analgesic effect. Clinical studies show that meloxicam is effective in treating rheumatoid arthritis and osteoarthritis. Compared with traditional anti-inflammatory drugs, meloxicam is safer, long-lasting, less side effects. In this thesis, the content of meloxicam was assayed by ultraviolet spectrophotometry method in preparations. High performance liquid chromatography was used to study the stability of meloxicam and to detect the related substances and degradation products of meloxicam. The results show that meloxicam maximum absorption at wavelength of 372nm. In the range of 0.580 ~ 0.869μg/ml, a good linear relationship isaccepted with, the availability of the regression equation: Y = 0.4785X +0.0138, R = 0.9996; U.S. Related substances and degradation products of meloxicam was determined by RP-HPLC, with the mobile phase 0.05mol / L ammonium dihydrogen phosphate (pH4.5) - methanol (47:53), the detection wavelength: 370nm. The results suggest that meloxicam is instable on acid, oxidants, light couditions and it is stable on alkali and heat couditions. Keywords：UV - Vis Spectrophotometry；HPLC；Meloxicam
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夫西地酸的分离纯化研究_制药工程论文范文
制药工程论文范文 目 录 中文摘要I 英文摘要II 目录III 1．绪论1 1.1夫西地酸概述2 1.2大孔吸附树脂概述2 1.3总结3 2．实验部分3 2.1夫西地酸标准品在不同溶剂下的溶解度实验3 2.2夫西地酸发酵液的分离研究4 2.3夫西地酸的HZ-816树脂柱层析提纯5 3．结果与讨论7 3.1夫西地酸标准品在不同溶剂下的溶解度实验7 3.2夫西地酸发酵液的分离研究结果7 3.3夫西地酸的HZ-816树脂柱层析提纯结果9 3.4实验结果总结12 4．总结与展望13 4.1总结13 4.2展望13 致谢15 参考文献16 摘要 :本论文研究建立夫西地酸发酵液的分离纯化工艺，夫西地酸是一种窄谱、高效的抗生素，对预防和治疗葡萄球菌感染疗效显著。根据夫西地酸的溶解特性，采用过滤、干燥、萃取和柱层析等技术相结合，设计不同的工艺路线，通过对酸碱调节试剂、流动相梯度、柱层析树脂的选择，采用高效液相色谱法检测产物的纯度和收率作为评价指标，得到的最优工艺流程。该工艺其产品的收率为44.53%，组分含量为98.23%。该方法简便易行，准确可靠，能有效控制产品的质量，可用于夫西地酸的工业生产。 关键词：夫西地酸；分离；柱层析；高效液相色谱法 Abstract:This study was to establish the technology process of the separation and purification of the fucidic acid. Fusidic acid is an efficient narrow-spectrum antibiotic. The treatment of staphylococcus aureus infection is remarkable. According to the dissolution characteristics of fusidic acid, we used the filtering, drying, extraction, column chromatography and so on to design different technics processes. HPLC was used to check the purity and yield of the products to optimize the production process of the fucidic acid. Results suggested that the yield was 44.53% and the component concentration was 98.23%. This technology process was simple, accurate, reliable and effective for the manufacturing production. Keywords: Fucidic acid; Separation; Column chromatography; HPLC
